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differentiation myogenic c2c12 cells  (ATCC)


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    ATCC differentiation myogenic c2c12 cells
    Differentiation Myogenic C2c12 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 8335 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/differentiation myogenic c2c12 cells/product/ATCC
    Average 99 stars, based on 8335 article reviews
    differentiation myogenic c2c12 cells - by Bioz Stars, 2026-06
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    Micrograph of the effects of differentiating medium and AR antagonists on mouse C2C12 myogenic cells. Shown are: (A) control differentiated myotubes and (B) cells after 4 day treatment with 5 μM of the adenine derivative 2 followed by maintaining the cells for an additional 7 days in differentiating medium containing 0.1 μM dexamethasone, 50 μg/mL ascorbic acid-2-phosphate and 10 mM β-glycerophosphate (see Materials and Methods). Cells treated identically to (B), except lacking differentiating medium are shown in (C). Arrows indicate cells that stained positive for alkaline phosphatase activity using a semiquantitative method. Bar in (A) indicates 20 μm.

    Journal: Journal of medicinal chemistry

    Article Title: ‘Reversine’ and its 2-Substituted Adenine Derivatives as Potent and Selective A 3 Adenosine Receptor Antagonists

    doi: 10.1021/jm050221l

    Figure Lengend Snippet: Micrograph of the effects of differentiating medium and AR antagonists on mouse C2C12 myogenic cells. Shown are: (A) control differentiated myotubes and (B) cells after 4 day treatment with 5 μM of the adenine derivative 2 followed by maintaining the cells for an additional 7 days in differentiating medium containing 0.1 μM dexamethasone, 50 μg/mL ascorbic acid-2-phosphate and 10 mM β-glycerophosphate (see Materials and Methods). Cells treated identically to (B), except lacking differentiating medium are shown in (C). Arrows indicate cells that stained positive for alkaline phosphatase activity using a semiquantitative method. Bar in (A) indicates 20 μm.

    Article Snippet: Studies of Cellular Differentiation Mouse C2C12 myogenic cells (ATCC, Manassas, VA) were plated in 6-well plates in growth medium (DMEM with 10% fetal bovine serum) and after overnight incubation (during which time cells attach to the bottom of the plate) 5 μM of the adenine derivative or other AR antagonists were added.

    Techniques: Control, Staining, Activity Assay